For the first round victory over Morecambe, manager Alex Neil made 11 changes to his side, but will not have that luxury against Leeds on Tuesday night.
The suspension of Paul Gallagher - the final game of his three match ban - plus injuries to skipper Tom Clarke and Alan Browne, along with the long-term casualties of Calum Woods, Sean Maguire and Billy Bodin, mean that options are not as prevalent for the Lilywhites boss.
Clarke is still feeling the affects of a groin issue, whilst Browne is to undergo a scan on hamstring injury Monday to assess the severity of the injury that kept him out of the trip to Pride Park at the weekend.
Chris Maxwell was used in between the sticks against the Shrimps and could get his second start of the season, whilst the likes of Josh Earl, Jordan Storey, Daniel Johnson, Louis Moult, Brandon Barker and Tom Barkhuizen, who were with Maxwell on the bench at Derby could also all get a start.
Young Irish midfielder Adam O'Reilly got on the bench for the first round victory and he may again be involved in the 18 for the trip across the M62 to Elland Road.
Leeds also made a number of changes for their first round win over Bolton Wanderers and with his side top of the Championship, Marcelo Bielsa may choose to do the same again.
Skipper Liam Cooper is currently on the sidelines after sustaining an injury in the warm up at Swansea City last Tuesday, but fellow central defender Pontus Jansson returned at the weekend.
Stuart Dallas and Adam Forshaw both missed the trip to Norwich City and will be assessed ahead of the visit of the Lilywhites, but the likes of summer signings Jack Harrison and Patrick Bamford are expected to come into the side for the game.
The influential Pablo Hernandez came off through injury against the Canaries at the weekend, but even if he is fit, it is unlikely the player will be risked against the Lilywhites.